Background:CCL22/MDC
Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.
原厂资料:
Product Summary:
The Quantikine Mouse MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MDC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MDC and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MDC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MDC.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse MDC spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=5)
91
82-100
Heparin Plasma (n=4)
90
80-109
Cell Culture Supernates (n=7)
102
87-112
EDTA Plasma (n=4)
94
89-98
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MDC in each matrix were diluted with Calibrator Diluent and then assayed.