Background:CCL20/MIP-3 alpha
Macrophage inflammatory protein 3 alpha (MIP-3 alpha), also known as LARC (liver and activation-regulated chemokine) and as exodus-1, is one of many novel CC chemokines identified through bioinformatics. MIP-3 alpha is distantly related to other CC chemokines (20 - 28% amino acid sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% amino acid sequence identity with mouse and human MIP-3 alpha, respectively.
原厂资料：
Product Summary:
The Quantikine Mouse MIP-3 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIP-3 alpha in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIP-3 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIP-3 alpha.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
23
23
23
Mean
10
43
118
11
40
112
Standard Deviation
0.6
1.7
5.9
1
3.6
7.5
CV%
6
4
5
9.1
9
6.7
Recovery:
The recovery of mouse MIP-3 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=4)
106
95-119
Heparin Plasma (n=4)
98
85-115
Cell Culture Supernates (n=4)
100
96-106
Linearity:
To assess the linearity of the assay, samples spiked with recombinant mouse MIP-3 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
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