Molecule Information:Angiopoietin-like Protein 3/ANGPTL3
Aliases:AGNPT5; Ang-5; Angiopoietin-5; ANGPTL3
Entrez Gene IDs: 27329 (Human); 30924 (Mouse)
Background:Angiopoietin-like 3
Angiopoietin-like 3 (ANGPTL3) is a liver secreted glycoprotein that is structurally related to the angiopoietins, although it does not bind Tie-1 or Tie-2. Full length ANGPTL3 circulates in the plasma as do its proteolytically cleaved coiled coil and fibrinogen-like domains. ANGPTL3 promotes an increase in circulating triglyceride levels by directly inhibiting lipoprotein lipase. ANGPTL3 expression is dysregulated in some strains of obese and diabetic mice.
原厂资料：
Product Summary:
The Quantikine Mouse ANGPT-L3 immunoassay is a 4.5 hour solid phase ELISA designed to measure ANGPT-L3 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ANGPT-L3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ANGPT-L3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ANGPT-L3.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of ANGPT-L3 spiked to levels throughout the range of the assay was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=4)
103
93-120
Linearity:
To assess the linearity of the assay, samples containing high concentrations of ANGPT-L3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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