Human ErbB3/Her3 Alexa Fluor 700 MAb (Clone 66223) (25 TESTS)
Human ErbB3/Her3 Alexa Fluor 700 MAb (Clone 66223) (25 TESTS)
产品编号：RD-FAB3481N-025 品牌：R&D
原厂货号：FAB3481N-025
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描述：
Species Reactivity
Human
Specificity
Detects human ErbB3/Her3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human EGF R is observed.
Source
Monoclonal Mouse IgG1 Clone # 66223
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Ser20-Thr643
Accession # P21860
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of ErbB3/Her3 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ErbB3/Her3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3481N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol forStaining Membrane-associated Proteins.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB3/Her3
ErbB3, also known as Her3 (Human Epidermal Growth Factor Receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the Heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of Phosphoinositide 3-Kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.
References:
Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
Detects human ErbB3/Her3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human EGF R is observed.
Source
Monoclonal Mouse IgG1 Clone # 66223
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Ser20-Thr643
Accession # P21860
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of ErbB3/Her3 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ErbB3/Her3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3481N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol forStaining Membrane-associated Proteins.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB3/Her3
ErbB3, also known as Her3 (Human Epidermal Growth Factor Receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the Heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of Phosphoinositide 3-Kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.
References:
Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
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![Flow Cytometry ErbB3/Her3 Antibody (66223) [Alexa Fluor® 700]](https://resources.rndsystems.com/images/datasheets/antibody/ErbB3_FAB3481N_Flow_Cytometry_16037.jpg)
