Mouse CD11b/Integrin alpha M Alexa Fluor 700 MAb (Cl M1/70) (25 TESTS)
Mouse CD11b/Integrin alpha M Alexa Fluor 700 MAb (Cl M1/70) (25 TESTS)
产品编号：RD-FAB1124N-025 品牌：R&D
原厂货号：FAB1124N-025
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描述：
Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha M/CD11b. Cross-reaction with human Integrin alpha M has been reported (1, 2).
Source
Monoclonal Rat IgG2B Clone # M1/70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Con A-activated C57BL/10 splenocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin alpha M/CD11b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Integrin alpha M/CD11b Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1124N) and Rat Anti-Mouse F4/80/EMR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5580P). Quadrant markers were set based on control antibody staining (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
References:
Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Entrez Gene IDs:
3684 (Human); 16409 (Mouse)
Alternate Names:
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6
原厂资料：
Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha M/CD11b. Cross-reaction with human Integrin alpha M has been reported (1, 2).
Source
Monoclonal Rat IgG2B Clone # M1/70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Con A-activated C57BL/10 splenocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin alpha M/CD11b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Integrin alpha M/CD11b Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1124N) and Rat Anti-Mouse F4/80/EMR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5580P). Quadrant markers were set based on control antibody staining (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
References:
Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Entrez Gene IDs:
3684 (Human); 16409 (Mouse)
Alternate Names:
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6
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![Flow Cytometry CD11b Antibody (M1/70) [Alexa Fluor® 700]](https://resources.rndsystems.com/images/datasheets/antibody/Integrin_alpha_M_FAB1124N_Flow_Cytometry_16113.jpg)
