Proteome Profiler Human Ubiquitin Array (1 KT)

产品编号：RD-ARY027 品牌：R&D 原厂货号：ARY027
产品分类：
应用分类：

包装：

运保温度:

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

Kit Summary

To detect changes in ubiquitinylation of 49 different proteins between samples. No specialized equipment is necessary

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are diluted and incubated with the Human Ubiquitin Array overnight. The array is washed to remove unbound proteins, followed by incubation with a biotinylated detection antibody. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the level of ubiquitinylation of the bound protein. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

4 Array Membranes
4-Well Multi-dish
Array Buffers
Wash Buffer
Detection Antibody Cocktail
Streptavidin-HRP
Chemiluminescent Detection Reagents
Transparency Overlay Template
Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8° C.

Simultaneously detect the Ubiquitination of these proteins in a single sample.
A20/TNFAIP3	ATF4	Bcl-2
beta-TrCP1/BTRC	Caspase-8	CBL
Cyclin D1/CCND1	CD44	cIAP-1/HIAP-2
cIAP-2/HIAP-1	COX-2/PTGS2	EGF R
ER-alpha/NR3A1	ErbB2	ErbB3
ErbB4	FASN	F-box protein 15/FBXO15
FBXW7/Cdc4	FGF R2	HGF R/c-met
HIF-1 alpha	HSP70	HSP90AA1/HSP90a
IGF-I R	I kappa B alpha	I kappa B eplison
IKK gamma/NEMO	Insulin R	IRAK1
IRF3	IRS1	M-CSF R
MSP R/Ron	Nrf2/NFE2L2	NIK/MAP3K14
p21CDKN1A/Cip1	p53	PDGF R alpha
PDGF R beta	RIP1	SCF R/c-Kit
TfR	TNF R1/TNFRSF1A	TRAF-2
TRAF-3	TRAF-6	Trk A
VEGF R3/Flt-4	Ubiquitin

Assays and antibodies for Analytes represented in the Human Ubiquitin Array Kit

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

原厂资料：

Kit Summary

To detect changes in ubiquitinylation of 49 different proteins between samples. No specialized equipment is necessary

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are diluted and incubated with the Human Ubiquitin Array overnight. The array is washed to remove unbound proteins, followed by incubation with a biotinylated detection antibody. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the level of ubiquitinylation of the bound protein. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

4 Array Membranes
4-Well Multi-dish
Array Buffers
Wash Buffer
Detection Antibody Cocktail
Streptavidin-HRP
Chemiluminescent Detection Reagents
Transparency Overlay Template
Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8° C.

Simultaneously detect the Ubiquitination of these proteins in a single sample.
A20/TNFAIP3	ATF4	Bcl-2
beta-TrCP1/BTRC	Caspase-8	CBL
Cyclin D1/CCND1	CD44	cIAP-1/HIAP-2
cIAP-2/HIAP-1	COX-2/PTGS2	EGF R
ER-alpha/NR3A1	ErbB2	ErbB3
ErbB4	FASN	F-box protein 15/FBXO15
FBXW7/Cdc4	FGF R2	HGF R/c-met
HIF-1 alpha	HSP70	HSP90AA1/HSP90a
IGF-I R	I kappa B alpha	I kappa B eplison
IKK gamma/NEMO	Insulin R	IRAK1
IRF3	IRS1	M-CSF R
MSP R/Ron	Nrf2/NFE2L2	NIK/MAP3K14
p21CDKN1A/Cip1	p53	PDGF R alpha
PDGF R beta	RIP1	SCF R/c-Kit
TfR	TNF R1/TNFRSF1A	TRAF-2
TRAF-3	TRAF-6	Trk A
VEGF R3/Flt-4	Ubiquitin

Assays and antibodies for Analytes represented in the Human Ubiquitin Array Kit

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.