描述:
Wnt proteins are cysteine-rich secreted glycoproteins that play critical roles in both
carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled
family in conjunction with a coreceptor of the lowdensity lipoprotein receptorrelated
protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. Downstream
effects of Wnt signaling occur through multiple pathways with differing intracellular
components: the canonical Wnt/βcatenin pathway, the Wnt/Ca2+pathway, and the
planar cell polarity (PCP) pathway. Wnt-5b is a 49 kDa glycoprotein that is implicated
in the Wnt/Ca2+ and PCP pathways. These pathways can inhibit canonical Wnt/βcatenin
signaling. Human and mouse Wnt5b are synthesized as 359 amino acid (aa) precursors that
contain a 17 aa signal sequence and a 342 aa mature region. Mature mouse Wnt-5b shares
94%, 98%, 90% and 88% aa identity with mature human, rat, chick and Xenopus Wnt-5b,
respectively. Although Wnt-5a and Wnt-5b share 83% aa identity, their effects may be
different. For example, Wnt-5b, but not Wnt-5a, promotes cell cycle progression and is
weakly transforming. Wnt-5a and -5b are thought to coordinate developmental events,
such as chondrocyte differentiation and formation of endochondral bone. In contrast to more
focused expression of Wnt-5a, Wnt-5b is constitutively expressed at low levels throughout
mouse embryonic development. In adult mice, Wnt-5b is widely expressed, including heart,
liver, brain, lung, testes, kidney, and pancreas. Wnt-5b is up-regulated during early
adipogenesis, and its overexpression in 3T3-L1 cells partially inhibits canonical Wnt
suppression of adipogenesis. It enhances PPARγ expression and promotes differentiation of
preadipocytes. Human Wnt5b polymorphisms have been associated with Type II diabetes.
原厂资料:
Wnt proteins are cysteine-rich secreted glycoproteins that play critical roles in both
carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled
family in conjunction with a coreceptor of the lowdensity lipoprotein receptorrelated
protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. Downstream
effects of Wnt signaling occur through multiple pathways with differing intracellular
components: the canonical Wnt/βcatenin pathway, the Wnt/Ca2+pathway, and the
planar cell polarity (PCP) pathway. Wnt-5b is a 49 kDa glycoprotein that is implicated
in the Wnt/Ca2+ and PCP pathways. These pathways can inhibit canonical Wnt/βcatenin
signaling. Human and mouse Wnt5b are synthesized as 359 amino acid (aa) precursors that
contain a 17 aa signal sequence and a 342 aa mature region. Mature mouse Wnt-5b shares
94%, 98%, 90% and 88% aa identity with mature human, rat, chick and Xenopus Wnt-5b,
respectively. Although Wnt-5a and Wnt-5b share 83% aa identity, their effects may be
different. For example, Wnt-5b, but not Wnt-5a, promotes cell cycle progression and is
weakly transforming. Wnt-5a and -5b are thought to coordinate developmental events,
such as chondrocyte differentiation and formation of endochondral bone. In contrast to more
focused expression of Wnt-5a, Wnt-5b is constitutively expressed at low levels throughout
mouse embryonic development. In adult mice, Wnt-5b is widely expressed, including heart,
liver, brain, lung, testes, kidney, and pancreas. Wnt-5b is up-regulated during early
adipogenesis, and its overexpression in 3T3-L1 cells partially inhibits canonical Wnt
suppression of adipogenesis. It enhances PPARγ expression and promotes differentiation of
preadipocytes. Human Wnt5b polymorphisms have been associated with Type II diabetes.