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描述:
The gene for CXCL1/GROα was initially discovered in hamster cells, using subtractive
hybridization techniques, as a message that is overexpressed in tumorigenic cells and
in normal cells during growth stimulation. The hamster cDNA was cloned and used as a
probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding
a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from
a human melanoma cell line and found to be identical to GRO. In addition to the initially
cloned GRO gene, now designated CXCL1, two additional GRO genes, GROβ or MIP2α and
GROγ or MIP-2β, which shared 90% and 86% amino acid sequence homology, respectively,
with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C)
subfamily of chemokines.
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