描述：
Wnt-3a is one of about 19 vertebrate members of the Winglesstype MMTV integration
site (Wnt) family of highly conserved cysteinerich secreted glycoproteins important for
normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction
with a coreceptor of the lowdensity lipoprotein receptorrelated protein family (LRP-5 or -6),
or the Ryk atypical receptor tyrosine kinase. Mouse Wnt-3a is a 44 kDa secreted hydrophobic
glycoprotein containing a conserved pattern of 24 cysteine residues. Like other Wnts, Wnt-3a
is modified by palmitate addition (at Cys 77) following glycosylation, which increases its
hydrophobicity, secretion and activity. A second site at Ser 209 is modified by palmitoleic
acid and also contributes to activity and secretion. Mouse Wnt3a shares 96% amino acid (aa)
identity with human Wnt3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a
precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa
63352. Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is
morphogen that is thought to coordinate somitogenesis and mesoderm boundary
determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a.
When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior
posterior patterning, somite development and tailbud formation. Recombinant Wnt-3a
promotes proliferation of committed stem cell lineages in vitro, and may help maintain the
cells in an undifferentiated state.For example, Wnt-3a can induce selfrenewal of hematopoietic
stem cells, allowing expansion without further differentiation.
原厂资料：
Wnt-3a is one of about 19 vertebrate members of the Winglesstype MMTV integration
site (Wnt) family of highly conserved cysteinerich secreted glycoproteins important for
normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction
with a coreceptor of the lowdensity lipoprotein receptorrelated protein family (LRP-5 or -6),
or the Ryk atypical receptor tyrosine kinase. Mouse Wnt-3a is a 44 kDa secreted hydrophobic
glycoprotein containing a conserved pattern of 24 cysteine residues. Like other Wnts, Wnt-3a
is modified by palmitate addition (at Cys 77) following glycosylation, which increases its
hydrophobicity, secretion and activity. A second site at Ser 209 is modified by palmitoleic
acid and also contributes to activity and secretion. Mouse Wnt3a shares 96% amino acid (aa)
identity with human Wnt3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a
precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa
63352. Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is
morphogen that is thought to coordinate somitogenesis and mesoderm boundary
determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a.
When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior
posterior patterning, somite development and tailbud formation. Recombinant Wnt-3a
promotes proliferation of committed stem cell lineages in vitro, and may help maintain the
cells in an undifferentiated state.For example, Wnt-3a can induce selfrenewal of hematopoietic
stem cells, allowing expansion without further differentiation.
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