This antibody is functionally tested against 20ng of an E. coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 1 minute exposure. This antibody has also been tested in Western blot against 25ng of recombinant Positope™ protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM).
Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.
This antibody has also been used successfully to immunoprecipitate fusion proteins that contain theV5 epitope.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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