Blue/White Cloning Qualified:Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
Genome Qualified:Promega's Genome-Qualified restriction enzymes are assayed to ensure optimal performance in genomic analysis applications.
Available at High Concentration:Cat.# R5104 contains 1,250 units of SgfI at a concentration of 40–80u/μl.
Notes
Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
Sensitive to CpG methylation.
SgfI is inactivated by freezing at –70°C.
Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.