ActinRed™ 555 ReadyProbes® Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes® reagents, ActinRed™ 555 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.
• High-affinity staining of F-actin with superior specificity compared to antibody methods • Outstanding fluorescence performance (Ex/Em: 540/565 nm) • Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette • Stability at room temperature—keep handy at your scope or cell culture area.
Cell imaging applications Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, ActinRed™ 555 reagent allows high-contrast discrimination of actin staining.
Suggestions for use • ActinRed™ 555 reagent may be added directly to fixed cells in full media or buffer solutions • In most cases 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained. • ActinRed™ 555 dye is excited with a maximum at 540 nm and has an emission maximum at 565 nm. It is detected through standard TRITC (Rhodamine/DiI/Cy3™/Alexa Fluor® 546) filters.
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For Research Use Only. Not for use in diagnostic procedures.
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