Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette, microinjection, or our Influx™ pinocytotic cell-loading reagent. The fluorescence signal from these cells is generally measured using fluorescence microscopy.
Calcium Indicator (Cell-Impermeant Salts) Specifications:
• Label (Ex/Em of Ca2+–bound form): Rhod-5N (551/576 nm)
• Fluorescence intensity increase upon binding Ca2+: >100 fold
• Kd for Ca2+ in the absence of Mg2+, in buffer: ~320 µM
• Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
Using TPEN to Control Heavy Metal Cations In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.
More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes® calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes® Handbook.
For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) reviewIndicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes® Handbook.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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