Thermo Scientific NBT is used to enhance blue color development during detection of alkaline phosphatase activity (see Protocols). It is suitable for immunohistochemistry and cytochemistry. NBT is also a substrate for oxidases and dehydrogenases.
Applications
Detection of alkaline phosphatase activity in:
Southern blotting
Western blotting
Northern blotting
Dot/slot blots
Plaque and colony screening
原厂资料:
Thermo Scientific NBT is used to enhance blue color development during detection of alkaline phosphatase activity (see Protocols). It is suitable for immunohistochemistry and cytochemistry. NBT is also a substrate for oxidases and dehydrogenases.
Applications
Detection of alkaline phosphatase activity in:
Southern blotting
Western blotting
Northern blotting
Dot/slot blots
Plaque and colony screening
注意事项:
Preparation of a 75 g/mL stock solution in 70% dimethylformamide is recommended.