The pALTER®-MAX Vector is a 5,534bp plasmid. It contains the human cytomegalovirus (CMV) immediate-early enhancer/promoter region for strong, constitutive expression of cloned DNA inserts in a variety of mammalian cell types. The pALTER®-MAX Vector as supplied is chloramphenicol-resistant and ampicillin-sensitive.
Features - Benefits
High Expression:Protein expression driven by the human cytomegalovirus (CMV) immediate-early enhancer/promoter region allows high constitutive expression in a variety of cell lines. A chimeric intron composed of the 5´-splice site from the β-globin intron and the 3´-splice site from an IgG intron allows increased expression levels.
Efficient:SV40 late polyadenylation signal for efficient RNA processing. T7 and T3 RNA polymerase promoters flank the multiple cloning site and allow RNA in vitro synthesis from sense or antisense strands.
Flexible:Restriction sites flanking the CMV enhancer (BglII and SgfI) and the CMV promoter (SgfI and I-PpoI) allow for easy replacement of these regions by alternative regulatory regions.