该引物的序列是:5`-d(TTACGCCAAGTTATTTAGGTGACA)-3`。pTARGET™ Sequencing Primer 溶于无菌水中,浓度为10ng/μl(1.25pmol/μl)。
原厂资料:
The pTargeT™ Sequencing Primer is designed for sequencing inserts cloned into the pTargeT™ Mammalian Expression Vector (Cat.# A1410). The sequencing primer hybridizes to the region of the lacZ gene at nucleotides 1367–1344 on the pTargeT™ Vector.
The primer can be used only for sequencing inserts cloned into the pTargeT™ Vector. The primer sequence is not a binding site for any RNA polymerases and cannot be used to generate in vitro transcripts.
The sequence of the pTargeT™ Sequencing Primer is 5´-d(TTACGCCAAGTTATTTAGGTGACA)-3´.
The primer is supplied at a concentration of 10ng/μl (1.25pmol/μl) in sterile water.