KINASE TRACER 222

• 产品编号：PV6121      品牌：invitrogen       原厂货号：PV6121
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

描述：

Kinase Tracer 222 is an Alexa Fluor® 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen® Eu Kinase Binding Assay.

The LanthaScreen® Eu Kinase Binding Assay
• Is easily optimized
• Measures inhibitor binding
• Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

 

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注意事项：

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.