Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure, and signals through binding to the obesity receptor (OB-R) in the hypothalamus.
Leptin is a recognised marker of obesity and human studies have shown a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
Target Species
Rat
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant leptin expressd in E.coli
Preservative Stabilisers
None present
Molecular Weight
16.2kD (147 amino acid residues)
Reconstitution
Reconstitute with 1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Purity
>98% by SDS PAGE and HPLC analysis
Activity
Determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5µg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
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