M-CSF (Macrophage colony-stimulating factor) is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.
Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling.
Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant M-CSF expressed in E.coli
Preservative Stabilisers
None present
Molecular Weight
36.4kD (156 amino acid homodimer)
Reconstitution
Reconstitute with 100ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Purity
%98 by SDS PAGE and HPLC analysis
Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
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