PMP44 is an E. coli-derived recombinant protein corresponding to full-length mature murine insulin-like growth factor 1 (IGF-1), a 70 amino acid peptide that is structurally and functionally related to insulin but with higher growth-promoting activities.
Recombinant mouse IGF-1 has an ED50 of <2.0ng/ml as determined by a cell proliferation assay using FDC-P1 cells.
Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 10 ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preservative Stabilisers
None present
Purity
>97% by SDS PAGE and HPLC
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Protein Molecular Weight
7.6kD (70 amino acid sequence)
Endotoxin Level
<0.1 ng/ug
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
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