Bone morphogenetic protein 13 (BMP-13), otherwise known as growth differentiation factor 6 (GDF6), is expressed in hypertrophic chondrocytes during embryonic development of long bones, and implicated as a regulator of the growth and maintenance of articular cartilage. BMP-13 is also expressed at the boundaries of developing carpals, tarsals, and vertebrae, and mutations in the BMP-13 gene, have been identified in both familial and sporadic cases of Klippel-Feil syndrome (KFS), a congenital disorder of spinal segmentation. Functional BMP-13 is a disulfide-linked homodimer consisting of two 120 amino acid polypeptide chains, derived by proteolytic processing of a biologically inactive precursor protein.
Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant BMP-13 expressed in E.coli
Preservative Stabilisers
None present
Molecular Weight
27.0kD (120 amino acid residue homodimer)
Reconstitution
Reconstitute with 500ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Purity
>95% by SDS PAGE and HPLC analysis
Activity
The ED50 was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells in the range of 2.0-3.0 µg/ml.