M-CSF (Macrophage colony-stimulating factor) is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.
Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation.
Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant M-CSF expressed in E.coli
Preservative Stabilisers
None present
Molecular Weight
36.8kD (159 amino acid homodimer)
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Purity
>98% by SDS PAGE and HPLC analysis
Activity
The ED50, determined by the dose-dependent stimulation of the proliferation of M-NSF-60 cells, was = 1 ng/ml corresponding to a specific activity of = 1 x 106 units/mg.
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