BDNF (brain-derived neurotrophic factor) is a potent neurotrophin and member of the NGF-beta family, secreted from dense core vesicles, which promotes the growth, differentiation and survival of neurons within, or directly connected to, the central nervous system (CNS).
BDNF signals through binding to the high affinity receptor trkB, and also binds to the low affinity neurotrophin receptor LNGFR. Studies have shown the involvement of BDNF in the modulation of short-term synaptic function and in the formation of long-term memory, and reduced levels of BDNF are seen in patients with Alzheimer's and Huntington disease.
Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant BDNF expressed in E.coli
Preservative Stabilisers
None present
Molecular Weight
27.0kD (119 amino acid homodimer)
Reconstitution
Reconstitute with 100ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Purity
>98% by SDS PAGE and HPLC analysis
Activity
Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0 µg/ml.
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