PHP130 is an E. coli-derived recombinant protein corresponding to amino acids 23-215 of human fibroblast growth factor 8 (FGF 8), a secreted peptide that promotes cell growth in an autocrine manner.
The peptide sequence contains an initiator methionine at the N-terminus and is homologous to the murine, canine and bovine sequences.
PHP130 has an ED50 of <0.5ng/ml as determined by dose-dependent thymidine uptake in BaF3 cells expressing FGF receptors.
Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Preparation
Recombinant protein expressed in Escherichia coli
Preservative Stabilisers
None present.
Protein Molecular Weight
22.4kD (193 amino acid sequence)
Reconstitution
Reconstitute with 25ul of 10mM TRIS pH 8.0.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Purity
>95% by SDS PAGE and HPLC analysis
Activity
2 x 106 units/mg
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml after reconstitution