Granulocyte CSF is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and differentiates certain myeloid leukemic cells. Human and Murine G-CSF share approximately 75% amino acid sequence homology and have biological cross-reactivity.
Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Preparation
Purified recombinant protein expressed in E.Coli
Preservative Stabilisers
None present
Protein Molecular Weight
18.7kDa (174 amino acid residues)
Reconstitution
Reconstitute with 100 ul distilled water
Purity
>98% by SDS PAGE and HPLC analysis
Activity
1 x 107 units/mg
Approx. Protein Concentrations
0.1 mg/ml
Buffer Solution
Lyophilised from 5mM Sodium Citrate pH4.0
Note
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Serotec recommend that the vial is gently mixed after reconstitution.