SNAP-tag Purified Protein

产品编号：P9312S 品牌：New England Biolabs 原厂货号：P9312S
产品分类：生化和细胞分析 > 标签、标记试剂盒 > 细胞标记试剂及试剂盒
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运保温度:

-80°C

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描述：

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O⁶-alkylguanine-DNA alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates. The coding sequence of SNAP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. SNAP-tag protein (MW: 19,694) was expressed and purified according to the instructions in the IMPACT™ kit manual (NEB #E6901). The purified SNAP-tag protein was dialyzed into 1X phosphate buffered saline (PBS) solution containing 1 mM DTT at 1 mg/ml (50 μM) and stored at -80°C.

Mass spectrometry analysis indicates the SNAP-tag purified protein has two species with molecular weights of 19,694 and 19,829 (with a C-terminal DTT moiety attached).