收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

RNase B

  • 产品编号:P7817S      品牌:New England Biolabs       原厂货号:P7817S
  • 产品分类:克隆 > DNA/RNA修饰酶 > RNase
  • 应用分类:
 
包装:
运保温度: -20°C
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

RNase B is a high mannose glycoprotein (1-3) that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. RNase B has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays.

Product Source

Bovine pancreas

Storage Conditions

50 μl Milli-Q water
pH 7.0 @ 25°C

Molecular Weights

Theoretical: 17000 daltons


注意事项:

  1. 250 μg is enough for approximately 20 reactions.
  2. RNase B Deglycosylation Protocol: 
    1. In a 10 µl reaction, add 2 µl of RNase B, 1 µl of 10X Glycoprotein Denaturing Buffer, and 5 µl of dH2O. 
    2. Boil for 10 minutes. 
    3. Add 1 µl of 10X G7 Reaction Buffer and 1 µl of 10% NP-40. 
    4. Add 1 µl of endoglycosidase. Incubate at 37°C for 1 hour. 
    5. Visualize reaction products on 10–20% SDS-PAGE gel.


相关图片

说明书

参考文献

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错