ColorPlus™ Prestained Protein Marker, Broad Range includes a mixture of purified proteins covalently coupled to colored dye that resolves to 8 bands of even intensity, including one orange and one green reference band for easy orientation when electrophoresed (1). The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker.
Storage Conditions
63.5 mM Tris-HCl
50 mM NaCl
40 mM DTT
1 mM Na2EDTA
10% Glycerol
0.01% Phenol red
2% SDS
pH 7.0 @ 25°C