Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). The apparent molecular weight of the prestained proteins are given in the table below. For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker
Storage Conditions
70 mM Tris-HCl
33 mM NaCl
40 mM DTT
1 mM EDTA
10% Glycerol
0.01% Phenol red
2% SDS
pH 6.8 @ 25°C