Casein Kinase I (CK1)

• 产品编号：P6030S      品牌：New England Biolabs       原厂货号：P6030S
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 蛋白激酶 > 胞质酪氨酸激酶
• 应用分类：

描述：

Casein Kinase I (CK1) is a serine/threonine protein kinase (1). It is a truncated monomer (1-317) of the CK1d isoform, which lacks the regulatory C-terminal domain, containing 111 amino acids (2). In vitrostudies have shown that the activity of CK1δ is regulated by autophosphorylation of its C-terminal domain. Autophosphorylation of this domain on potential sites leads to inhibition of kinase activity (3). There are at least seven mammalian CK1 isoforms and their splice variants, and distinct CK1 family members have a variety of roles in eukaryotic cells (4).  
 

Highlights

• Protein serine/threonine kinase
• Rat, recombinant (E. coli)

Product Source

Isolated from a strain of E. coli that carries a clone expressing CK1 δ derived from a rat testis cDNA library (kindly provided by Dr. P. J. Roach). Two codons, Ser-318 and Arg-319, have been changed to stop codons, resulting in a truncation of the C-terminal portion of the expressed protein (2).

Recognition Determinant

The most effective recognition motif for phosphorylation by CK1 is pSXXS/T where Ser in the position -3 is phosphorylated (3). Also, the clusters of 3 or 4 acidic residues ending at the position -3, preferably Asp, can specify phosphorylation by CK1. However, the substrates so formed are much poorer than those containing phosphate groups (5).

原厂资料：