pHrodo® Red AM Intracellular pH Indicator is a novel fluorogenic probe used to measure intracellular pH in live cells. pHrodo® Red is weakly fluorescent at neutral pH but increasingly fluorescent as the pH drops. This reagent can quantify cellular cytosolic pH in the range of 9-4 with a pKa of ~6.5 with excitation/emission of 560/585 nm. pHrodo® Red is photostable and appropriate for use in multiplexing experiments with green fuorophores such as GFP, FITC, and Alexa Fluor® 488.
This pHrodo® Red dye has been modified with AM ester groups, which results in an uncharged molecule that can permeate cell membranes. Once inside the cell, the lipophilic blocking groups are cleaved by nonspecific esterases, resulting in a compound that is retained within the intracellular space. Fluorescence intensity of the probe is then an indicator of intracellular pH. Subsequent use of the Intracellular pH Calibration Buffer Kit (Cat. No. P35379) allows this intracellular pH to be quantified.
pHrodo® Red AM Intracellular pH Indicator is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometry, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos®, FLoid™, Tali™, and Attune™ instruments.
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For Research Use Only. Not for use in diagnostic procedures.
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