PHRODO RED C2 MALEIMIDE 1 MG

产品编号：P35371 品牌：invitrogen 原厂货号：P35371
产品分类：生化和细胞分析 > 标签、标记试剂盒 > 硫醇反应荧光团，生物素及其他标记
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Store at -20°C

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描述：

The thiol-reactive pH-sensitive pHrodo® Red Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo® Red dramatically increases fluorescence as the pH of its surroundings become more acidic.
• Use pH-sensitive pHrodo® Red Maleimide to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with green fluorescent markers such as GFP, pHrodo® Green, and many others

The increase in fluorescence of pHrodo® Red as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo® Red makes it useful for multi-color experiments. pHrodo® Red has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo® Red in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.

pHrodo® Red Maleimide is a thiol-reactive dye that can be used to create pHrodo® Red bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use.

pHrodo® Red is also available in an amine-reactive form (see pHrodo® Red SE), as well as a selection of ready-to-use conjugates (e.g., E. coli, S. aureus, and dextran). In addition, pHrodo® Green reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties.

注意事项：

For Research Use Only. Not for use in diagnostic procedures.