New pH-sensitive pHrodo® dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo® dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.
• Specific detection of phagocytosis and endocytosis • Reduced signal variability and improved timing in sensitive experiments. • Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo® dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-madepHrodo® E.coli BioParticles® conjugates in imaging or flow applications, orpHrodo® SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.