T7 RiboMAX? Express RNAi System

产品编号：P1700 品牌：promega 原厂货号：P1700
产品分类：克隆 > 体外转录 > 体外转录
应用分类：

包装：

50 × 20μl reactions

运保温度:

–20°C

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其它组分：

RNase A Solution【子货号：A797D，包装：1 x 200μl，，运保温度：–20°C,stored at 22–25°C after the initial thaw】

Sodium Acetate, 3.0M (pH 5.2)【子货号：P135A，包装：1 x 1ml，，运保温度：–20°C】

Enzyme Mix, T7 Express【子货号：P132A，包装：1 x 100μl，，运保温度：–20°C】

Nuclease-Free Water【子货号：P119A，包装：1 x 1,250μl，，运保温度：–20°C】

RiboMAX™ Express T7 2X Buffer【子货号：P164A，包装：1 x 500μl，，运保温度：–20°C】

pGEM® Express Positive Control Template【子货号：P256A，包装：1 x 5μg，，运保温度：–20°C】

RQ1 RNase-Free DNase【子货号：M610C，包装：1 x 110u，，运保温度：–20°C】

描述：

说明

T7 RiboMAX™ Express RNAi System (T7 RiboMAX™ Express RNA 干扰系统) 是一种体外转录系统，为短时间内产生毫克级的双链RNA(dsRNA) 而设计。产生的dsRNA 中不含蛋白和其它成分的污染，适合于哺乳动物和非哺乳动物系统的RNA干扰(RNAi)研究。
T7 RiboMAX™ Express RNAi System 可用于合成哺乳动物系统实验中的21bp 的短干扰RNAs(siRNAs)。体外合成的siRNAs 已被证明能够与化学合成的siRNAs 一样有效，均可在哺乳动物细胞中诱导RNA 干扰。
此外, T7 RiboMAX™ Express RNAi System 还可合成将近200bp 或更长的dsRNA 分子，可被应用于非哺乳动物系统。两条互补的RNA 链是以DNA 为模板来合成的( 模板可以是质粒或PCR 产物)。产生的RNA 链在转录反应后退火形成dsRNA。反应中残存的单链RNA 和DNA 模板会以核酸酶消化去除。然后以异丙醇沉淀来纯化dsRNA ，得到的dsRNA 可被用于选择进行RNAi 实验的生物体。

特点

• 节省时间：T7 RiboMAX™ Express RNAi System 可在30 分钟内产生毫克级RNA。

• 将移液的误差减至最低：4 种rNTPs 和2X 转录缓冲液已经混合，将移液误差和组装反应组分的时间减到最小。

原厂资料：

The T7 RiboMAX™ Express RNAi System is an in vitro transcription system designed for producing milligram amounts of double-stranded RNA (dsRNA) in a short amount of time. The dsRNA is free of protein and other contaminants and is suitable for use in RNA interference (RNAi) in both mammalian and nonmammalian systems.

The T7 RiboMAX™ Express RNAi System can be used to synthesize short interfering RNAs (siRNAs) of 21bp for use in mammalian systems. siRNAs synthesized in vitro have been demonstrated to be as effective as chemically synthesized siRNAs for inducing RNAi in mammalian cells.
In addition, the T7 RiboMAX™ Express RNAi System can be used for the synthesis of dsRNA molecules of approximately 200bp or greater, which can be applied to nonmammalian systems. Two complementary RNA strands are synthesized from DNA template (either plasmid or PCR product). The resulting RNA strands are annealed after the transcription reaction to form dsRNA. Any remaining single-stranded RNA and DNA template are removed with a nuclease digestion step. The dsRNA is then purified by isopropanol precipitation and can be introduced into the organism of choice for RNAi applications.

Features - Benefits

Save Time: The T7 RiboMAX™ Express RNAi System produces milligram amounts of RNA in as little as 30 minutes.
Minimize Pipetting Errors: The four rNTPs and 2X transcription buffer have been combined, thus minimizing pipetting errors and setup time.

Applications

Synthesis of siRNA duplexes for mammalian RNA interference.
Generation of large quantities of long dsRNA for nonmammalian RNA interference.
For more information, see the Protocols & Applications Guide.

References

Yu, J.-H. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 6047–52.
Donze, O. and Picard, D. (2002) Nucl. Acids Res. 20, e46.

注意事项：

储存条件

储存于-20℃。