T7 RiboMAX? Express Large Scale RNA Production System

产品编号：P1320 品牌：promega 原厂货号：P1320
产品分类：克隆 > 体外转录 > 体外转录
应用分类：

包装：

1 system

运保温度:

-20℃

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其它组分：

pGEM® Express Positive Control Template【子货号：P256A，包装：2 x 5μg，，运保温度：-20℃】

RQ1 RNase-Free DNase【子货号：M610C，包装：1 x 110u，，运保温度：-20℃】

Nuclease-Free Water【子货号：P119A，包装：1 x 1.25ml，，运保温度：-20℃】

Enzyme Mix, T7 Express【子货号：P132A，包装：1 x 100μl，，运保温度：-20℃】

Sodium Acetate, 3.0M (pH 5.2)【子货号：P135A，包装：1 x 1ml，，运保温度：-20℃】

RiboMAX™ Express T7 2X Buffer【子货号：P164A，包装：1 x 500μl，，运保温度：-20℃】

描述：

说明

T7 RiboMAX^™ Express Large Scale RNA Production System(T7 RiboMAX^™ 快速大量RNA 制备系统) 是为在短时间内稳定表达毫克级RNA 而设计的体外转录系统。由于优化了酶混合液和转录缓冲液，30 分钟内RNA 产量可达5-8.5mg/ml，而其它同类产品则需要2-4 小时。为了尽量减少移液的次数和误差，在2× 转录缓冲液中就包括了所有四种rNTP。另外，系统还包含RQ1 RNase-Free DNase (RQ1 无RNA 酶的DNA 酶)，用于转录后除去质粒模板。

由于2× 缓冲液和四种rNTP 已经混合在一起，我们不建议用T7 RiboMAX^™Express System 合成需要加帽的RNA。如要合成加帽RNA，请订购常规的RiboMAX^™ Large Scale RNA Production System-T7(Cat.# P1300)。

特点

• 快速：T7 RiboMAX^™ Express System 仅需30 分钟就能制备出毫克级RNA，而其它同类产品需要2-4 小时。

• 方便：四种rNTP 和2× 转录缓冲液已经混合好，可减少移液误差和实验时间。

• 灵活：有效转录各种大小的DNA 模板。转录子可短至21bp。

原厂资料：

The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro transcription system designed for the consistent production of milligram amounts of RNA in a short amount of time. Due to optimization of the enzyme mix and transcription buffer, yields of 5–8.5mg/ml are generated in 30 minutes, compared to 2–4 hours with other commercially available systems. To minimize pipetting steps and errors, the 2X transcription buffer includes all four rNTPs. In addition, the system includes RQ1 RNase-Free DNase for the removal of plasmid template after transcription.

Due to the combined 2X buffer and rNTPs, the T7 RiboMAX™ Express System is not recommended for the synthesis of RNA for applications that require capped RNA. For synthesis of capped RNA, please order the standard RiboMAX™ Large Scale RNA Production System—T7 (Cat.# P1300).

Features - Benefits

Fast: The T7 RiboMAX™ Express System produces milligram amounts of RNA in as little as 30 minutes rather than 2–4 hours as with other commercially available systems.
Convenient: The four rNTPs and 2X transcription buffer have been combined, thus minimizing pipetting errors and setup time.
Flexible: Efficiently transcribes DNA templates of varying sizes. Works with transcripts as short as 21bp.

Applications

Generation of large quantities of uncapped transcripts for in vitro or in vivo translation.
In vitro synthesis of tRNA, rRNA, RNA virus genomes and ribozymes.
Production of substrates for RNA splicing, RNA secondary structure, antisense RNA and RNA:protein interaction studies.
Synthesis of long RNAs for nonmammalian RNA interference.

The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro transcription system designed for consistent production of milligram amounts of RNA in a short amount of time. This is about 10- to 20-fold more RNA than is produced in a standard Riboprobe® System transcription reaction. T7 RiboMAX™ Express System reactions differ from Riboprobe® System reactions in three primary ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer, NTP and magnesium concentrations are elevated and inorganic pyrophosphatase is included in the reaction.

注意事项：

储存条件

储存于-20℃。