Sodium Orthovanadate (Vanadate)

产品编号：P0758L 品牌：New England Biolabs 原厂货号：P0758L
产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 蛋白抑制剂、酶抑制剂
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-20°C

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描述：

Sodium Orthovanadate (Vanadate, Na₃VO₄) is a commonly used general inhibitor for protein phosphotyrosyl phosphatases (PTPs). It is a competitive inhibitor. The inhibition by Vanadate is completely reversible upon the addition of EDTA or by dilution. Vanadate is activated for maximal inhibition of PTPs following the procedure described by J.A.Gordon (1).

The PTP activities are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride (NEB# P0759) and EDTA. Routinely Vanadate is used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays (1, 2).

注意事项：

Common buffer components such as EDTA and reducing agents may interact with Vanadate, affecting its potency (2).

100% of inhibition of TC PTP (NEB #P0752) is found when NEBuffer for PTP (NEB #B0760S) is supplemented with 5 mM Vanadate.There is no inhibition of the same PTP in the presence of 50 mM Fluoride under the same condition (3).

Storage: Sterile purified water adjusted to pH 10.0 (1,2).