No chromatography required Solulink’s R-PE-Antibody Conjugation Kit offers an innovative catalyzed conjugation based on Solulink’s proprietary linking technology that delivers greater than 95% conversion of antibody to conjugate. The kit includes everything needed to make two direct-labeled R-PE antibody conjugates from 500 µg of any user-supplied antibody.
S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker, an NHS-ester, reacts with lysine residues, incorporating HyNic functional groups (hydrazinonicotinamide) onto the antibody.
S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to R-PE, providing a pre-activated, highly fluorescent R-PE called 4FB-R-PE.
HyNic-modified antibody is incubated with pre-activated 4FB-R-PE in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds.
Catalyzed conjugation The efficiency of catalyzed conjugation greatly simplifies conjugate purification. Solulink’s proprietary linking technology is catalyzed by the TurboLink™ catalyst, aniline, under mild reaction conditions, increasing the rate and efficiency, leading to quantitative conversion of free antibody to conjugate. The complete absence of free antibody at the end of the catalyzed reaction leaves only two components; excess R-PE and conjugate.
R-PE-Antibody Conjugation Kit Advantages
Catalyzed Conjugation – Fast catalyzed method generates conjugates quickly and efficiently
Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
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