Oregon Green® 514 phalloidin is a high-affinity F-actin probe conjugated to green fluorescent Oregon Green® 514 dye.
• Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 511/528 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples • pH insensitive under physiological conditions
Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green® 514 provides green fluorescence with brightness superior to fluorescein. Oregon Green® 514 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green® 514 phalloidin allows high-contrast discrimination of actin staining.
Use in Multiple Applications Oregon Green® 514 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green® 514 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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