NuPAGE® Novex Bis-Tris Gels provide the best separation and resolution of small- to medium-sized proteins by utilizing a neutral pH environment which minimizes protein modifications. These high-performance gels have a one-year shelf life when stored at +4°C to 25°C, eliminating the waste of throwing out expired gels. Use NuPAGE® Novex Bis-Tris Gels for protein sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. The NuPAGE® System also provides the most efficient means for transferring proteins to membranes for subsequent analysis.
Formulation: The NuPAGE® System is based upon a Bis-Tris-HCl buffered (pH 6.4) polyacrylamide gel, with a separating gel that operates at pH 7.0. While NuPAGE® Novex Bis-Tris Gels do not contain SDS, they are formulated for denaturing gel electrophoresis applications only.
Recommended Buffers: NuPAGE® Novex Bis-Tris Gels are available in three acrylamide concentrations: 10%, 4-12%, and 12%. By combining any of these three gel types with the NuPAGE® MES or MOPS Buffers, six separation ranges can be obtained. The NuPAGE® MES Buffer is recommended for resolving small proteins, while NuPAGE® MOPS Buffer is for resolving medium to large size proteins. Use of NuPAGE® LDS Sample Buffer and NuPAGE® Sample Reducing Agent will ensure complete sample reduction. For transfer, NuPAGE® Transfer Buffer is recommended.
Platform:
XCell6™ MultiGel,
XCell SureLock™ Mini,
ZOOM® IPGRunner™ Mini
Regulatory Statement:
For Research Use Only. Not for use in diagnostic procedures.
System:
ZOOM®
Gel Size:
Mini (8 cm X 8 cm)
Gel Type:
Bis-Tris Gel
Quantity:
10 gels
Thickness:
1.0 mm
Well Format:
IPG Well
Product Size:
1 box
Gel Percentage:
4-12 %
Number of Wells:
2D well (IPG)
Separation Range:
15 kDa to 260 kDa (MOPS Buffer),
3.5 kDa to 160 kDa (MES Buffer)
Mode of Separation:
Molecular Weight
Sample Loading Volume:
7 cm strips
内容和储存
Catalog numbers for gels refer to a single box. One box contains 10 gels. Store at +4°C to 25°C. Shelf life is 12 months.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.