10%Bis-Tris预制胶，1mm, 10孔，Mini(8cm x 8cm)

• 产品编号：NP0301BOX      品牌：invitrogen       原厂货号：NP0301BOX
• 产品分类：蛋白质检测与分析 > 蛋白质凝胶电泳 > 预制蛋白胶 > Bis-Tris胶
• 应用分类：

描述：

NuPAGE® Novex® 10% Bis-Tris 凝胶 (1.0-mm 厚, 10-孔) 是预制的聚丙烯酰胺凝胶，在变性条件下，对小分子量到中等分子量的蛋白质具有极佳的分离效果。 与传统的Tris-甘氨酸 凝胶不同，NuPAGE® Novex® Bis-Tris 凝胶的中性pH环境，能最大程度减少蛋白质变性。用NuPAGE® Novex Bis-Tris 凝胶制备的完整的蛋白质，对于蛋白质测序，质谱以及其他技术领域具有重要作用。同样的，用NuPAGE® 制备的凝胶也可以满足日常蛋白质分离要求。NuPAGE® Novex® Bis-Tris 凝胶有两种尺寸以及多种孔型构造。 每盒有10张凝胶。

• 快速的运行时间—35分钟即可完成电泳
• 更长的有效期—NuPAGE® Novex® Bis-Tris 凝胶在室温条件可保存至少1年时间
• 更好的蛋白质完整性—已经过优化的样品制备过程，可保护蛋白质的完整性。
• 多种分子量分离范围—选择合适的凝胶和电泳缓冲液，获得最佳的分离效果

原厂资料：

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use. 

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins. 

We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using theiBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using theXCell II Blot Module or the Mini Blot Module.

Related links 
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

注意事项：

For Research Use Only. Not for use in diagnostic procedures.