NuPAGE® Novex Bis-Tris Gels provide the best separation and resolution of small- to medium-sized proteins by utilizing a neutral pH environment which minimizes protein modifications. These high-performance gels have a one-year shelf life when stored at +4°C to 25°C, eliminating the waste of throwing out expired gels. Use NuPAGE® Novex Bis-Tris Gels for protein sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. The NuPAGE® System also provides the most efficient means for transferring proteins to membranes for subsequent analysis.
Formulation: The NuPAGE® System is based upon a Bis-Tris-HCl buffered (pH 6.4) polyacrylamide gel, with a separating gel that operates at pH 7.0. While NuPAGE® Novex Bis-Tris Gels do not contain SDS, they are formulated for denaturing gel electrophoresis applications only.
Recommended Buffers: NuPAGE® Novex Bis-Tris Gels are available in three acrylamide concentrations: 10%, 4-12%, and 12%. By combining any of these three gel types with the NuPAGE® MES or MOPS Buffers, six separation ranges can be obtained. The NuPAGE® MES Buffer is recommended for resolving small proteins, while NuPAGE® MOPS Buffer is for resolving medium to large size proteins. Use of NuPAGE® LDS Sample Buffer and NuPAGE® Sample Reducing Agent will ensure complete sample reduction. For transfer, NuPAGE® Transfer Buffer is recommended.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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