The NuPAGE® Antioxidant is a proprietary reagent that helps maintain proteins in a reduced state during protein gel electrophoresis. The NuPAGE® Antioxidant is added to the running buffer in the upper (cathode) buffer chamber only when performing protein gel electrophoresis under reducing conditions (with samples that were previously reduced using DTT or another reducing agent). The NuPAGE® Antioxidant migrates with reduced proteins during gel electrophoresis, preventing the proteins from reoxidizing and keeping the proteins in a reduced state. The NuPAGE® Antioxidant also protects sensitive amino acids such as methionine and tryptophan from oxidizing. The NuPAGE® Antioxidant is NOT compatible with gel systems other than the NuPAGE® system, as the antioxidant is not efficient at the higher pHs of other gel systems; furthermore, it may NOT be used in place of a sample reducing agent.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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