pACP-ADRβ2 Control Plasmid

产品编号：N9321S 品牌：New England Biolabs 原厂货号：N9321S
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > DNA亚克隆载体
应用分类：

包装：

20 μg

运保温度:

-20°C

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

This control plasmid contains the gene encoding the Beta-2 Adrenergic Receptor cloned as a fusion to the C-terminus of the ACP-tag. The signal peptide fused to the N-terminus of ACP-tag is based on the 5HT3A Serotonin Receptor. The Beta-2 Adrenergic Receptor is a member of the G protein coupled receptors and mediates the catecholamine-induced activation of adenylate cyclase through the action of G proteins.

The ACP-Beta-2 Adrenergic Receptor is inserted in the plasma membrane with the ACP-tag exposed to the extracellular side of the membrane. When labeled with CoA substrates, it gives a selective cell membrane fluorescence labeling pattern.The full sequence and map for pACP-ADRβ2 can be downloaded here.

The ACP-tag is a small protein tag (8 kDa) based on the acyl carrier protein (ACP). It allows the specific, covalent attachment of virtually any molecule to a protein of interest. ACP-tag substrates are derivatives of coenzyme A (CoA). In the labeling reaction, the substituted phosphopantetheine group of CoA is covalently attached to a conserved serine residue of the ACP-tag by a phosphopantetheine transferase (ACP or SFP Synthase). Having no cysteines, the ACP-tag is particularly suited for specifically labeling cell-surface proteins, and should be useful for labeling secreted proteins with disulfide bridges such as antibodies.

There are two steps to using this system: subcloning and expression of the protein of interest as an ACP-tag fusion, and labeling of the fusion protein with the CoA substrate of choice. The cloning and expression of ACP-tag protein fusions is described in documents provided with the pACP-tag(m)-2 cloning plasmid. The labeling of fusion proteins with CoA substrates is described in the documentation supplied with CoA substrates and ACP or SFP Synthase.

注意事项：

Storage: pACP-ADRβ2 is supplied in TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) at a concentration of 0.5 µg/µl. Plasmid solutions can be stored at 4°C for up to one week. For long-term storage -20°C is recommended.