pSNAPf-Cox8A Control Plasmid

• 产品编号：N9185S      品牌：New England Biolabs       原厂货号：N9185S
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > DNA亚克隆载体
• 应用分类：

描述：

This control plasmid contains the gene encoding the Cytochrome C oxidase, subunit 8-2 (COX8-2) protein cloned upstream of the SNAP_f coding sequence in pSNAP_f, as a fusion to the N- terminus of the SNAP-tag. Cytochrome C oxidase is located in the inner mitochondrial membrane and is the terminal enzyme of the respiratory chain. The COX8-2-SNAP_f fusion protein gives mitochondrial fluorescence when labeled with SNAP-Cell™ substrates. The full sequence and map for pSNAP_f-Cox8A can be downloaded.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on human O⁶-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

pSNAP_f-Cox8A contains an improved version of SNAP-tag, termed SNAP_f. SNAP_f displays faster kinetics in in vitro labeling and fast, specific and efficient labeling in live and fixed cell applications, thereby rendering it a desired research tool for analysis of protein dynamics.

There are two steps to using this system: sub cloning and expression of the protein of interest as a SNAP_f fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of the SNAP_f-Cox8A fusion protein is described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAP-tag substrates.

原厂资料：

注意事项：

Storage: pSNAP_f-Cox8A is supplied in TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) at a concentration of 0.5 µg/µl. Plasmid solutions can be stored at 4°C for up to one week. For long-term storage -20°C is recommended.