pSNAPf Vector

• 产品编号：N9183S      品牌：New England Biolabs       原厂货号：N9183S
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
• 应用分类：

描述：

pSNAP_f Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells. This plasmid encodes SNAP_f, a SNAP-tag protein, which is expressed under control of the CMV promoter. The expression vector has an IRES (internal ribosome entry site) and a neomycin resistance gene downstream of the SNAP_f for the efficient selection of stable transfectants. pSNAP_f Vector contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP_f.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O⁶-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

pSNAP_f contains an improved version of SNAP-tag, termed SNAP_f. SNAP_f displays faster kinetics in in vitro labeling and fast, specific and efficient labeling in live and fixed cell applications, thereby rendering it a desired research tool for analysis of protein dynamics.

 

There are two steps to using this system: sub cloning and expression of the protein of interest as a SNAP_f fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP_f fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with the SNAP-tag substrates.

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