pGLuc Mini-TK 2 is a cloning vector for mammalian cells, containing a minimal promoter fragment from the HSV thymidine kinase (TK) promoter adjacent to a reporter gene, the secreted luciferase from the copepodGaussia princeps. GaussiaLuciferase (GLuc) is a 20 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). The pGLuc Mini-TK 2 Vector contains a multiple cloning site (MCS) upstream of the minimal TK promoter for cloning promoter or enhancer elements. A neomycin resistance gene under the control of the SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
Product Source
Isolated fromE. colistrain NEB 10-beta by a standard DNA purification procedure.