pTK-GLuc Vector

产品编号：N8084S 品牌：New England Biolabs 原厂货号：N8084S
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
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描述：

The pTK-GLuc Vector is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive Herpes Simplex Virus thymidine kinase promoter. Gaussia Luciferase (GLuc) is a 20 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). pTK-GLuc has a multiple cloning site (MCS) between the GLuc stop codon and the polyadenylation site. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.

Highlights

• TK promoter: 18–771

• GLuc coding: 802–1359

•Start codon: 802–804

•Stop codon: 1357–1359

•Signal peptide: 802–852

•Multiple cloning site (MCS) downstream of GLuc: 1360–1391 (NotI, AgeI, XhoI, XbaI)

•SV40 late polyadenylation signal: 1392–1622

•Neo promoter (SV40): 2215–2550

•Neomycin resistance gene 2602–3396

•Neo^R poly-A(SV40 early): 3570–3700

•Bacterial replication ori (pMB1) 4730–4142

•Amp resistance 5761–4901