Description:
pTWIN1 is an E. coli expression vector which can be used with the IMPACT™ Kit (NEB #E6901). pTWIN vectors are designed for protein purification or for the isolation of proteins with an N-terminal cysteine and/or a C-terminal thioester (1). A polylinker in the vector is designed for the in-frame fusion of a target gene between the modified Ssp DnaB (2) and Mxe GyrA inteins (3,4). The presence of the chitin binding domain from Bacillus circulans (5,6) facilitates purification. The double-stranded vector is 7,375 base pairs in length.
Source:
pTWIN1 contains two mini-inteins, one derived from the Synechocystis sp DnaB intein (154 amino acids) (7) and the other from the Mycobacterium xenopi GyrA intein (198 amino acids) (8).
Concentration:
200 μg/ml
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
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