pTYB4 Vector

• 产品编号：N6704S      品牌：New England Biolabs       原厂货号：N6704S
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 细菌表达载体
• 应用分类：

描述：

Description: 
pTYB4 is an E. coli expression vector (7,474 bp) used in the IMPACT™ Kit (NEB #E6901) (1). This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa) (1,2). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein (3). 

For fusion of the N-terminus of the target protein to the intein tag, use pTYB11 (NEB #N6901) or pTYB12 (NEB #N6902).

Source:
pTYB4 is isolated from an E. coli strain (r- m-) by a standard plasmid purification procedure.
 

原厂资料：