pKLCF-c Vector

• 产品编号：N3745S      品牌：New England Biolabs       原厂货号：N3745S
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 酵母表达载体
• 应用分类：

描述：

• PLAC4-PBI promoter does not express in E. coli allowing toxic genes to be cloned prior to their expression in yeast.

• Universal MCS lies downstream of DNA encoding CBD and PLAC4-PBI promoter.

• Acetamidase expression for non-antibiotic selection in K. lactis.

• Ampicillin resistance for propagation in E. coli.

• Permits expression of CBD-tagged fusion proteins and their one-step purification directly from growth medium

Description:

Vector pKLCF-c contains the strong K. lactis PLAC4-PBI promoter (1), DNA encoding theK. lactis Cts1p chitin-binding domain (2), a universal multiple cloning site (MCS), the K. lactis LAC4 transcription terminator (TT), and a fungal acetamidase selectable marker gene (amdS) expressed from the yeast ADH1 promoter (PADH1). An E. coli replication origin (ori) and ampicillin resistance gene (ApR) is present for propagation of pKLCF-c inE. coli. SacII or BstXI linearized pKLCF-c integrates into the LAC4 locus of the K. lactisgenome upon transformation of K. lactis competent cells.

Source:
pKLCF-c is isolated from E. coli strain ER2268 by a standard DNA purification procedure.

Concentration:
1,000 μg/ml

Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
 

原厂资料：